|
Diddugodage Chamila Jeewani1,2*, Wang Zhonghua1 , Jian
Wenhui1 , Muththanthirige Don Lalith Chandana Nishantha1,
Nan Wenzhi1 and Liu Tianxiang1
1State Key Laboratory of Crop Stress Biology for Arid
Areas, College of Agronomy, Northwest A&F University,
Yangling, Shaanxi, China
2Grain Legumes and Oil Crops
Research and Development Centre, Department of Agriculture,
Angunakolapelessa, Sri Lanka
Abstract
Blue grained wheat contained a higher
quantity of natural anthocyanin compounds while normal
commercial wheat does not have. Though the genes related to
several colours of wheat have been identified, the major
genes in blue wheat are still unrevealed. Hence, combining
the SNP mapping, and transcriptome analysis, pivotal genes
regulating blue grain trait was identified. SNP genotyping
was carried out in an F2 blue and white wheat population.
The blue trait was controlled by a gene/locus located
between two SNP markers of IWB 18525 and IWB16381 on the 4D
chromosome. Comparative transcriptome analysis revealed that
40 structural differentially expressed genes (DEGs) related
to anthocyanin biosynthesis had significant expression
differences between blue and non-blue samples. Among them,
12 DEGs expressed only in blue samples while 2 DEGs were
specific to blue wheat. Only two F3’5’H genes located in 4D
(Traes_4DL_27C195FDE, Traes_4DL_5A3D8F519) were consistent
with the location results performed in SNP genotyping.
Further, F3’5’H is considered as the main enzyme for
Delphinidin compounds, cause for blue colouration. Hence,
two genes encoding F3’5’H in the 4D chromosome
preferentially account for the blue pigmentation in wheat.
Keywords:
Anthocyanin, Blue wheat, F3’5’H, SNP genotyping, Structural
genes, Transcriptome
Full Text : pdf
(1397kb)
* - Corresponding Author
|
